Lecture 9. Gene expression technologies (03.05.2003)

    Gene expression technologies
  1. Introduction to DNA microarrays. Microarrays in overview.
  2. Array fabrication. Preparation of labeled probes. Microarray hybridization.
  3. Reading methods. Imperfect data and noise.
  4. Generation and analysis of expression data. Normalization methods.
  5. SAGE, microplates, and differential display.
    Seminar’s discussions
  • Ink-jet spotting of arrays: Agilent
  • What is noise?
  • Affymetrix GeneChipTM glass slide experiments
    Main reading
  1. P.S. Meltzer. Large-scale genome analysis // BIOINFORMATICS: A Practical Guide to the Analysis of Genes and Proteins / A.D. Baxevanis, B.F. Francis Ouellette – 2001 John Wiley & Sons, Inc. – P. 393-41
  2. DNA Microarrays : A Practical Approach / Edited by Mark Schena. - Oxford University Press, 1999.
    Supplementary reading
  1. A Practical Approach to Microarray Data Analysis / Daniel P. Berrar ,Werner Dubitzky ,Martin Granzow. – Kluwer Academic Publishers, 2002

Internet Resources

DDD
NCBI Gene Expression Omnibus
SAGEmap
xProfiler
Cancer Genome Anatomy Project (CGAP)
CGAP Digital Differential Display (DDD)
CGAP xProfiler
Serial Analysis of Gene Expression (SAGE)
SAGE virtual Northern